

Software Version	3.16.10



Experiment File Path:	C:\Users\bnext\Documents\Biotek\experiments\20251120-104507-cytation5-pure-timecourse-gfp-.xpt
Protocol File Path:	C:\Users\bnext\Documents\Biotek\protocols\cytation5_protocols\cytation5-pure-timecourse-gfp.prt



Plate Number	Plate 1
Date	11/20/2025
Time	10:45:07 AM
Reader Type:	Cytation5
Reader Serial Number:	1705168
Reading Type	Reader

Procedure Details

Plate Type	Greiner 384 SV NoBind AutoMap
Well Selection	Runtime
Eject plate on completion	
Set Temperature	Setpoint 37C, Gradient 0 C
Start Kinetic	Runtime 6:00:00 (HH:MM:SS), Interval 0:05:00, 73 Reads
    Read	GFP-G70
	Fluorescence Endpoint
	Full Plate
	Filter Set 1
	    Excitation: 485/20,  Emission: 528/20
	    Optics: Top,  Gain: 70
	Light Source: Xenon Flash,  Lamp Energy: High,  Standard Dynamic Range
	Read Speed: Normal,  Delay: 100 msec,  Measurements/Data Point: 10
	Read Height: 10.5 mm
    Read	GFP-Gext
	Fluorescence Endpoint
	Full Plate
	Filter Set 1
	    Excitation: 485/20,  Emission: 528/20
	    Optics: Top,  Gain: extended
	Light Source: Xenon Flash,  Lamp Energy: High,  Extended Dynamic Range
	Read Speed: Normal,  Delay: 100 msec,  Measurements/Data Point: 10
	Read Height: 10.5 mm
End Kinetic	
Set Temperature	Incubator off

Layout
	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24
A																									Well ID
B																									Well ID
C																									Well ID
D																									Well ID
E																									Well ID
F																									Well ID
G	SPL1	SPL2	SPL3	SPL4																					Well ID
H	SPL5	SPL6	SPL7	SPL8																					Well ID
I																									Well ID
J																									Well ID
K																									Well ID
L																									Well ID
M																									Well ID
N																									Well ID
O																									Well ID
P																									Well ID

GFP-G70:485,528

Time	T GFP-G70:485,528	G1	G2	G3	G4	H1	H2	H3	H4
0:00:00	37.0	167	30	7	12	92587	70384	84987	64486
0:05:00	37.0	200	35	6	10	80452	61890	72234	54004
0:10:00	37.0	190	33	15	0	73144	59800	68546	51157
0:15:00	37.0	196	35	9	1	66177	58909	66907	52914
0:20:00	37.0	207	35	16	10	57709	58492	66739	57156
0:25:00	37.0	191	40	17	17	47015	58462	66941	57670
0:30:00	37.0	192	38	17	4	37979	58145	66356	58037
0:35:00	37.0	189	39	15	7	29761	58358	66630	59042
0:40:00	37.0	208	25	15	9	22754	58510	67007	59542
0:45:00	37.0	198	27	17	2	16858	58946	67181	59769
0:50:00	37.0	192	33	11	9	12664	59435	67295	60287
0:55:00	37.0	192	27	3	9	9126	59716	67294	60179
1:00:00	37.0	198	35	5	11	6755	60837	67720	60755
1:05:00	37.0	193	29	6	17	4943	61280	67268	60053
1:10:00	37.0	187	29	19	14	3761	61651	67562	60460
1:15:00	37.1	195	32	8	14	2984	62127	67907	60512
1:20:00	36.9	191	32	16	17	2363	61718	67654	60250
1:25:00	37.0	193	24	16	6	1994	62086	67761	60464
1:30:00	37.0	187	36	16	10	1744	62582	68117	60358
1:35:00	37.1	179	33	9	11	1537	62716	68063	60734
1:40:00	37.0	183	35	4	2	1446	62597	67828	60814
1:45:00	37.0	188	27	10	14	1372	62813	68277	60696
1:50:00	37.1	183	33	4	10	1339	63005	68137	60833
1:55:00	37.1	191	22	13	16	1281	62791	68494	60878
2:00:00	37.0	197	25	17	18	1270	63099	68450	60979
2:05:00	37.0	182	31	17	14	1236	62421	68797	60886
2:10:00	37.0	181	34	13	10	1220	62720	68759	61103
2:15:00	37.0	193	21	12	17	1227	62730	68793	61215
2:20:00	37.1	185	33	16	2	1206	62732	69100	60996
2:25:00	37.0	201	27	6	0	1224	63014	68794	61197
2:30:00	37.1	186	31	7	6	1215	63364	69157	61342
2:35:00	37.0	187	22	6	19	1214	63282	69034	61372
2:40:00	37.1	187	23	10	10	1218	62880	69567	61405
2:45:00	37.0	186	22	3	18	1208	62963	69688	61585
2:50:00	37.0	190	26	15	13	1213	63224	69311	61595
2:55:00	37.0	178	25	8	13	1208	63359	69729	61358
3:00:00	37.0	186	26	25	13	1221	63700	69696	61521
3:05:00	37.0	182	34	3	0	1200	63772	69657	61782
3:10:00	37.0	183	42	18	7	1189	63493	69792	61553
3:15:00	37.0	190	33	0	15	1196	63679	69923	61732
3:20:00	37.0	190	43	13	16	1196	63980	70153	61976
3:25:00	37.0	194	34	8	0	1216	63858	70399	61822
3:30:00	37.0	180	33	12	10	1213	63673	70893	62366
3:35:00	37.0	184	39	15	11	1217	63658	70598	61976
3:40:00	37.0	192	27	9	18	1214	63783	70769	61937
3:45:00	37.0	188	32	11	12	1226	63932	70413	61891
3:50:00	37.1	190	26	12	3	1211	64158	71052	62593
3:55:00	37.0	188	19	13	5	1215	64287	71363	62550
4:00:00	37.1	186	24	4	10	1213	64384	71108	62201
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GFP-Gext:485,528

Time	T GFP-Gext:485,528	G1	G2	G3	G4	H1	H2	H3	H4
0:00:06	37.0	1419	318	106	93	840891	639728	771629	592673
0:05:06	37.1	1611	319	78	71	733830	577710	655628	499301
0:10:06	37.0	1670	307	86	73	665564	546537	620630	470819
0:15:06	37.1	1674	291	75	70	596712	537442	607802	499271
0:20:06	37.0	1635	285	77	85	520069	535627	607305	520247
0:25:06	37.0	1638	278	69	80	425298	534702	608947	520235
0:30:06	36.9	1614	298	78	57	314618	537765	605884	529148
0:35:06	37.0	1632	281	79	76	246717	534636	604253	538622
0:40:06	37.0	1606	307	87	75	187940	537976	607233	543515
0:45:06	37.0	1642	278	83	78	140969	542019	607127	542402
0:50:06	37.1	1616	253	71	80	104734	552345	611485	544215
0:55:06	37.0	1642	257	78	70	76397	553218	610289	544538
1:00:06	37.1	1609	272	80	67	56176	556310	615593	545941
1:05:06	37.0	1579	257	92	75	41030	561017	614945	542840
1:10:06	37.0	1566	256	81	71	31292	563860	610729	547004
1:15:06	37.0	1569	251	80	70	24454	568760	617970	550772
1:20:06	37.0	1613	259	75	73	19888	567318	614869	548246
1:25:06	37.0	1618	262	73	83	16619	569283	615675	549367
1:30:06	37.0	1604	252	77	82	14551	570592	617799	547497
1:35:06	37.0	1548	254	87	71	13026	572166	619060	551427
1:40:06	37.0	1610	249	72	74	12226	570557	619826	552522
1:45:06	37.0	1545	261	82	64	11551	573996	620414	552999
1:50:06	37.1	1535	255	61	64	11102	573496	621748	551432
1:55:06	37.1	1509	241	66	67	10791	576884	619808	552613
2:00:06	37.0	1543	258	75	64	10704	576008	622264	554249
2:05:06	37.0	1553	224	72	72	10563	576770	621872	553359
2:10:06	37.0	1608	246	72	66	10450	576240	626701	554787
2:15:06	37.0	1599	254	73	77	10282	577868	623649	550723
2:20:06	37.1	1578	245	70	88	10354	574030	623529	556648
2:25:06	37.0	1622	259	68	82	10133	574413	629331	553709
2:30:06	37.0	1604	264	83	65	10273	580763	628970	557832
2:35:06	37.0	1559	237	86	75	10204	583108	626659	554102
2:40:06	37.0	1551	248	76	71	10324	580671	629186	554377
2:45:06	37.0	1571	248	69	78	10239	581989	631275	559778
2:50:06	37.0	1608	224	89	70	10152	580009	633265	562065
2:55:06	37.0	1518	240	72	72	10310	583127	633450	558695
3:00:06	37.0	1561	267	63	80	10180	585315	634091	556473
3:05:06	37.0	1568	229	75	66	10199	585100	633705	557971
3:10:06	37.0	1552	236	74	67	10220	587301	635234	562519
3:15:06	37.1	1573	229	74	75	10301	586950	634981	562175
3:20:06	37.0	1559	248	60	80	10371	587116	639023	560852
3:25:06	37.1	1577	270	76	68	10315	583746	638615	562353
3:30:06	37.0	1523	238	73	71	10423	592506	643592	564830
3:35:06	37.1	1601	239	81	75	10253	589836	641051	562643
3:40:06	36.9	1557	231	77	79	10362	587864	643060	561892
3:45:06	37.1	1513	248	74	70	10125	589366	646660	564969
3:50:06	37.0	1569	225	89	72	10420	586081	648949	563339
3:55:06	37.0	1543	248	85	69	10313	594112	646806	567540
4:00:06	37.0	1591	251	89	82	10390	591842	650807	565174
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